PERK polyclonal, anti-human

Quantity: 100 µg

Background:
EIF2AK3(Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3), also called PEK, is an enzyme that in humans is encoded by the EIF2AK3 gene. By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al.(1999) mapped the EIF2AK3 gene to chromosome 2p12. Harding et al.(2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called Perk, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress. Using a library of endoribonuclease-prepared short interfering RNAs(esiRNAs), Kittler et al.(2004) identified 37 genes required for cell division, one of which was EIF2AK3.

Description:
Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) detection. Tested with WB, ICC in Human.

Synonyms:
DKFZp781H1925 antibody, E2AK3_HUMAN antibody, EC 2.7.11.1 antibody, EIF2AK3 antibody, Eukaryotic translation initiation factor 2 alpha kinase 3 antibody, Eukaryotic translation initiation factor 2-alpha kinase 3 antibody, Heme regulated EIF2 alpha kinase antibody, HRI antibody, HsPEK antibody, Pancreatic eIF2 alpha kinase antibody, Pancreatic eIF2-alpha kinase antibody, PEK antibody, PRKR like endoplasmic reticulum kinase antibody, PRKR-like endoplasmic reticulum kinase antibody, WRS antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human PERK(163-176aa QWDQDRESMETVPF).

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.