PERK Polyclonal Antibody

Background:
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism

Synonyms:
DKFZp781H1925, E2AK3, EC 2.7.11.1, Eif2ak3, Eukaryotic translation initiation factor 2 alpha kinase 3, Eukaryotic translation initiation factor 2-alpha kinase 3, Heme regulated EIF2 alpha kinase, HRI, HsPEK, Pancreatic eIF2 alpha kinase, Pancreatic eIF2-alpha kinase, PEK, PRKR like endoplasmic reticulum kinase, PRKR-like endoplasmic reticulum kinase, WRS

Immunogen: Synthesized peptide derived from the N-terminal region of human PERK

Swissprot: Q9NZJ5

Calculated MW: 125 kDa
Observed MW: 125 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol pH 7.4.

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:40000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0201986