Phakinin polyclonal, anti-human, bovine

Quantity: 25 µl

Background:
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this BFSP2 gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Mutations in this gene have been associated with juvenile-onset, progressive cataracts and Dowling-Meara epidermolysis bullosa simplex.

Synonyms:
BFSP2, CP47, CP49, LIFL-L, CTRCT12, PHAKOSIN

Isotype: Guinea pig Polyclonal (GP63)

Immunogen:
Phakinin purified from calf lens

Form: Stabilized Serum

Specificity:
Specific detection of phakinin (Mr 47,000 polypeptide), intermediate filament protein detected in lens. Specific note: the antiserum cross react with filensin (115kD polypeptide also present in lens).

Reactivity: human, bovine

Applications: Immunohistochemistry on frozen sections, Immunoblotting (western)

Working DIlution: 1:100 for immunohistochemistry; 1:5,000 for immunoblotting (western, ECL)

Storage: 2-8C

References:
- Merdes, A. et al. (1993) J. Cell Biol. 123: 1507-1516
- Gounari, F. et al. (1993) J. Cell Biol. 121: 847-853