Phospho-AKT(T450) Polyclonal Antibody

Background:
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Research Area: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signaling transduction

Synonyms:
AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT1

Immunogen: A synthetic phosphorylated peptide around T450 of human AKT1 (NP_005154.2).

Swissprot: P31749
Gene ID: 207

Calculated MW: 48 kDa/55 kDa
Observed MW: 60 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:1000 IF 1:20-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.