Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody

Background:
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively.

Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction

Synonyms:
PRKAA1, AMPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA2, AMPK,

Immunogen: Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172

Swissprot: Q13131,P54646

Calculated MW: 62 kDa
Observed MW: 63 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:40000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.