Phospho-CHEK2 (Thr68) Polyclonal Antibody

Background:
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog (S. pombe), CHK2 checkpoint homolog, CHK2, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2

Immunogen: Synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68

Swissprot: O96017

Calculated MW: 61 kDa
Observed MW: 61 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:50-1:200, ELISA 1:10000-1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.