Phospho-γH2A.X(S139) Polyclonal Antibody

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
H2A.X, H2A/X, H2AX, Histone H2AX, H2AFX, histone H2AX, gamma H2A.X, γH2AX

Immunogen: A synthetic phosphorylated peptide around S139 of human γH2A.X (NP_002096.1).

Swissprot: P16104
Gene ID: 3014

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "IHC 1:50-1:100 IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.