Phospho-GSK3 beta (Ser9) Polyclonal Antibody

Background:
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.

Research Area: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction, Stem Cells

Synonyms:
Glycogen Synthase Kinase 3 Beta, Glycogen synthase kinase-3 beta, GSK 3 beta, GSK-3 beta, GSK3B, GSK3B, GSK3beta isoform, Serine/threonine-protein kinase GSK3B

Immunogen: Synthesized peptide derived from human GSK3β around the phosphorylation site of Ser9

Swissprot: P49841

Calculated MW: 47 kDa
Observed MW: 48 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300, ELISA 1:5000-1:20000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Alternative for product with catalog number:
sc-8257/sc-9166

Citation(s):
https://www.sciencedirect.com/science/article/pii/S0753332217330597
https://pubmed.ncbi.nlm.nih.gov/27889772/