Phospho-MDM2(S166) Polyclonal Antibody

Background:
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling

Synonyms:
MDM2, ACTFS, HDMX, hdm2

Immunogen: A synthetic phosphorylated peptide around S166 of human MDM2 (NP_002383.2).

Swissprot: Q00987
Gene ID: 4193

Calculated MW: 11-14 kDa/24-55 kDa
Observed MW: 95 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:1000 IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.