Phospho-PAK1 (Thr212) Polyclonal Antibody

Background:
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Research Area: Cancer, Cell Biology, Signal Transduction

Synonyms:
ADRB2, Alpha PAK, Alpha-PAK, MGC130000, MGC130001, p21 activated kinase 1, p21 protein (Cdc42/Rac) activated kinase 1, p21-activated kinase 1, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related), p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), p65 PAK, p65-PAK, P68-PAK, PAK alpha, PAK-1, Pak1, PAK1, Paka, PAKalpha, Protein kinase MUK2, Rac/p21-activated kinase, Serine/threonine-protein kinase PAK 1, STE20 homolog yeast

Immunogen: Synthesized peptide derived from human PAKα around the phosphorylation site of Thr212

Swissprot: Q13153

Calculated MW: 61 kDa
Observed MW: 65 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.