Phospho-POLR2A(S5) Polyclonal Antibody

Background:
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1, Pol II

Immunogen: A phospho specific peptide corresponding to residues surrounding S5 of human POLR2A

Swissprot: P24928
Gene ID: 5430

Calculated MW: 63 kDa/217 kDa
Observed MW: 260 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "
WB 1:500-1:2000
IHC 1:50-1:100
IF 1:50-1:100"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.