Phospho-RAF1(S259) Polyclonal Antibody

Background:
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Research Area: Cancer, Cell Biology, Metabolism, Signal Transduction

Synonyms:
CMD1NN, CRAF, NS5, Raf-1, c-Raf, RAF1

Immunogen: A synthetic phosphorylated peptide around S259 of human RAF1 (NP_002871.1).

Swissprot: P04049
Gene ID: 5894

Calculated MW: 73 kDa/75 kDa
Observed MW: 73 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.