Phospho-SMAD2/3 (Thr8) Polyclonal Antibody

Background:
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Research Area: Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells

Synonyms:
SMAD2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD3, MADH3, Mothers against decapentaplegic

Immunogen: Synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8

Swissprot: Q15796,P84022

Calculated MW: 48 kDa
Observed MW: 48 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, ELISA 1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://pubmed.ncbi.nlm.nih.gov/32508664/