Phosphotyrosine (P-Tyr) (clone SPM102), anti-all species
€0.00
In stock
SKU
ARP-24-MSM1X-232
Catalog Number: 24-MSM1X-232
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2b
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2b
Decription:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Synonyms: P-Tyr
Clone: SPM102
Isotype: Mouse IgG2b
Immunogen: Phosphotyrosine conjugated to KLH
Reactivity: all species
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunoprecipitation (1-2ug/500ug protein Lysate)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7, MDA-231, T47-D cells or breast carcinoma
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Synonyms: P-Tyr
Clone: SPM102
Isotype: Mouse IgG2b
Immunogen: Phosphotyrosine conjugated to KLH
Reactivity: all species
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunoprecipitation (1-2ug/500ug protein Lysate)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7, MDA-231, T47-D cells or breast carcinoma
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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