Phosphotyrosine polyclonal, anti-human, mouse, rat

Background:
Tyrosine phosphorylation plays a key role in cellular signaling. Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals. Antibodies specific for phospho-tyrosine have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

Application Dilution:
WB:1:50-1:200
FC:1:10-1:50
ICC:1:50

Specificity: Phosphotyrosine polyclonal antibody detects endogenous levels of Phosphotyrosine protein.

Immunogen:
Synthetic peptide conjugated to Keyhole Limpet Haemocyanin (KLH)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.