PI3 Kinase, p85 (C-terminal region) (clone M253), anti-human, mouse, rat, chicken

Background:
Phosphoinositide 3-kinase (PI3K) phosphorylates phosphatidylinositol (PI), PI-4-phosphate and PI-4,5-bisphosphate to catalyze the production of PI-3,4,5-triphosphate. Growth factors and hormones activate PI3K to coordinate various cellular events, such as cell growth, cell cycle entry, cell migration and cell survival. This PI3K activation is reversed by PTEN. In cancers caused by the loss of PTEN activity, PI3K is constituitively active and promotes cell proliferation. PI3Ks are composed of a catalytic subunit and a regulatory subunit. Various isoforms of the catalytic subunit (p110alpha, p110beta and p110delta) associate with regulatory subunits (p85alpha and p85beta) with the exception of p110gamma which interacts with a unique p101 regulatory subunit.

Immunogen: Clone M253 was generated from a recombinant protein corresponding to amino acids in the C-terminal region of human PI3 Kinase α subunit. This region is homologous to similar regions in rat and mouse PI3 Kinase α subunit.

Specificity: This antibody detects an 85 kDa* band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells.

Buffer/Storage:
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.