Pig BNP (Brain Natriuretic Peptide) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Natriuretic Peptide(BNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Natriuretic Peptide(BNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Natriuretic Peptide(BNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Natriuretic Peptide(BNP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cardiovascular biology, Neuro science, Hormone metabolism

Alternative Names:
GC-B, B-Type Natriuretic Peptide, Ventricular Natriuretic Peptide, Gamma-brain natriuretic peptide, Brain natriuretic peptide 32

Sample Type:
serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only