Pig IL8Rb (Interleukin 8 Receptor Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Beta(IL8Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Beta(IL8Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Beta(IL8Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Beta(IL8Rb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Tumor immunity, Infection immunity, Dermatology

Alternative Names:
CD182, CDw128b, IL8R-B, IL8-RB, CMKAR2, CXCR2, IL8R2, C-X-C chemokine receptor type 2, GRO/MGSA receptor, High affinity interleukin-8 receptor B

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 50 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only