PKA R2 (Phospho-S99) polyclonal, anti-human, mouse, rat, pig

Background:
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Alternative Name:
PRKAR2A, cAMP dependent protein kinase regulatory subunit alpha 2, cAMP dependent protein kinase regulatory subunit RII alpha, cAMP dependent protein kinase type II alpha regulatory chain, cAMP dependent protein kinase type II alpha regulatory subunit, cAMP-dependent protein kinase type II-alpha regulatory subunit, KAP2, KAP2, MGC3606, PKR 2, PKR2, PRKA R2, PRKAR 2, PRKAR2, PRKAR2A, Protein kinase A RII alpha subunit, Protein kinase cAMP dependent regulatory type II alpha,

Application Dilution:
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: PKA R2 (Phospho-S99) polyclonal antibody detects endogenous levels of PKA R2 protein only when phosphorylated at S99.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2

MW: 51 kDa

Swiss-Prot: P13861(Human) P12367(Mouse) P12368(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.