PKAα/β cat (phospho-T197) polyclonal, anti-human, mouse, rat

PKAα/β cat (phospho-T197) polyclonal, anti-human, mouse, rat

€328.00
In stock
SKU
BS4345
Catalog Number: BS4345
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC

Datasheet
Background:
PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.

Alternative Name:
cAMP-dependent protein kinase catalytic subunit alpha, PKA C-alpha, PRKACA, PKACA, PKA Ca, cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB, PKACB, PKA Cb,

Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200

Specificity: p-PKAα/β cat (T197) polyclonal antibody detects endogenous levels of PKAα/βcat protein when phosphorylated at Thr197.

Immunogen:
Synthetic phosphopeptide derived from human PKAα around the phosphorylation site of Threonine 197.

MW: ~ 42 kDa

Swis Prot.: P17612/P22694

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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