PKC γ (T214) polyclonal, anti-human, mouse, rat

Background:
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, λ, ν, μ, ι, and θ).

Alternative Name:
Protein kinase C gamma type, PKC-gamma, PKCG, PRKCG

Application Dilution:
WB: 1:500~1:1000

Specificity: PKC γ (T214) polyclonal antibody detects endogenous levels of PKC γ protein.

Immunogen:
Synthetic peptide, corresponding to amino acids 181-230 of Human PKC γ.

MW: ~ 80 kDa

Swiss-Prot: P05129

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.