PKC gamma polyclonal, anti-rat, human, mouse

Quantity: 100 µg

Background:
The gamma isotype of protein kinase C(PKC gamma) is a member of the classical PKC(cPKC) subfamily which is activated by Ca(2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain. Within the brain, PKC gamma is the most abundant in the cerebellum, hippocampus and cerebral cortex, where the existence of neuronal plasticity has been demonstrated. PKC gamma gene is mutated in spinocerebellar ataxia type 14(SCA14). Verbeek et al.(2005) point out the specific alterations in mutant PKC gamma function that could lead to the selective neuronal degeneration of SCA14.

Description:
Rabbit IgG polyclonal antibody for Protein kinase C gamma type (PRKCG) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
KPCG_HUMAN antibody, MGC57564 antibody, OTTHUMP00000067291 antibody, PKC-gamma antibody, PKCC antibody, PKCG antibody, PRKCG antibody, Protein kinase C gamma antibody, Protein kinase C gamma polypeptide antibody, Protein kinase C gamma type antibody, Protein kinase C, gamma antibody, SCA 14 antibody, SCA14 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human PKC gamma(665-685aa IDQADFQGFTYVNPDFVHPDA), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the C-terminus of human PKC gamma(665-685aa IDQADFQGFTYVNPDFVHPDA), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: rat.
Predicted to work with: human, mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.