PKC iota polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Protein kinase C iota type, also called PKCI, is an enzyme that in humans is encoded by the PRKCI gene. This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. PRKCI is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKCI is also a hedgehog target gene that forms a positive feedback loop with GLI and exists at increased levels in basal cell carcinomas.

Description:
Rabbit IgG polyclonal antibody for Protein kinase C iota type (PRKCI) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
aPKC lambda/iota antibody, aPKC-lambda/iota antibody, Atypical protein kinase C lambda/iota antibody, Atypical protein kinase C-lambda/iota antibody, DXS1179E antibody, KPCI_HUMAN antibody, MGC26534 antibody, nPKC iota antibody, nPKC-iota antibody, nPKCiota antibody, PKC lambda antibody, PKCI antibody, PKCiota antibody, PKClambda antibody, PRKC I antibody, PRKC iota antibody, PRKC lambda/iota antibody, PRKC-lambda/iota antibody, PRKCI antibody, Protein kinase C iota antibody, Protein kinase C iota type antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human PKC iota recombinant protein (Position: D21-Q214). Human PKC iota shares 96% and 97% amino acid (aa) sequence identity with mouse and rat PKC iota, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.