PKC zeta Polyclonal Antibody

Background:
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Research Area: Cancer, Signal Transduction

Synonyms:
14-3-3-zetaisoform, AI098070, aPKCzeta, C80388, EC 2.7.11.13, KPCZ, nPKC zeta, nPKC-zeta, OTTHUMP00000001368, OTTHUMP00000044160, PKC 2, PKC ZETA, PKC2, Pkcz, PKCZETA, PKM-zeta, included, PRKCZ, Protein kinase C zeta, Protein kinase C zeta form, Protein kinase C zeta type, r14-3-3, R74924, zetaPKC

Immunogen: Synthesized peptide derived from human PKC ζ around the non-phosphorylation site of Thr560.

Swissprot: Q05513

Calculated MW: 68 kDa
Observed MW: 80 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
http://journals.sagepub.com/doi/abs/10.1177/1010428317701442