PKCα (Phospho-T638) polyclonal, anti-human, mouse, rat

Background:
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca²⁺. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Alternative Name:
PKC-A, PKC-alpha, PRKCA, AAG6, Aging associated gene 6, aPKC, KPCA, PKC alpha, PKC-A, PKC-alpha, PKCA, PRKACA, PRKCA, Protein Kinase C alpha, Protein kinase C alpha type,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:50-1:500
IHC:1:50-1:500

Specificity: PKCα (Phospho-T638) polyclonal antibody detects endogenous levels of PKCα protein only when phosphorylated at T638.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

MW: 77 kDa

Swiss-Prot: P17252(Human) P20444(Mouse) P05696(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.