PKCβ polyclonal, anti-human, mouse

Background:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Alternative Name:
PKC-B, PRKCB, PKC-beta, PKCB, PRKCB1, KPCB, PKC beta, PKC-B, PKC-beta, PKCB, Prkcb, PRKCB I, PRKCB1, PRKCB2, Protein kinase C beta, Protein kinase C beta type, protein kinase C, beta 1 polypeptide, protein kinase C, beta-1,

Application Dilution:
WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: PKCβ polyclonal antibody detects endogenous levels of PKCβ protein.

Immunogen:
Recombinant protein

MW: 77 kDa

Swiss-Prot: P05771(Human) P68404(Mouse)

Purification & Purity:
Protein affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.