PKCζ (Phospho-T560) polyclonal, anti-human, mouse, rat

Background:
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, Ω, h, θ, λ/ι, ? and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Alternative Name:
PRKCZ, PKC2, 14-3-3-zetaisoform, AI098070, aPKCzeta, C80388, EC 2.7.11.13, KPCZ, nPKC zeta, nPKC-zeta, OTTHUMP00000001368, OTTHUMP00000044160, PKC 2, PKC ZETA, PKC2, Pkcz, PKCZETA, PKM-zeta, included, PRKCZ, Protein kinase C zeta, Protein kinase C zeta form, Protein kinase C zeta type, r14-3-3, R74924, zetaPKC,

Application Dilution:
WB:1:1,000-1:5,000
IHC:1:50-1:200

Specificity: PKCζ (Phospho-T560) polyclonal antibody detects endogenous levels of PKCζ protein only when phosphorylated at T560.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr560 of human PKC zeta.

MW: 68 kDa

Swiss-Prot: Q05513(Human) Q02956(Mouse) P09217(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.