PKM2 polyclonal, anti-human

Quantity: 100 µg

Background:
PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Description:
Rabbit IgG polyclonal antibody for Pyruvate kinase isozymes M1/M2 (PKM) detection. Tested with WB, IHC-P in Human.

Synonyms:
CTHBP antibody, Cytosolic thyroid hormone binding protein antibody, Cytosolic thyroid hormone-binding protein antibody, KPYM_HUMAN antibody, MGC3932 antibody, OIP 3 antibody, OIP-3 antibody, Oip3 antibody, OIP3 antibody, OPA interacting protein 3 antibody, Opa-interacting protein 3 antibody, p58 antibody, PK Muscle type antibody, PK, muscle type antibody, PK2 antibody, Pk3 antibody, PKM antibody, PKM2 antibody, PYKM antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase 3 antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase, muscle 2 antibody, TCB antibody, THBP1 antibody, Thyroid hormone binding protein 1 antibody, Thyroid hormone binding protein cytosolic antibody, Thyroid hormone-binding protein 1 antibody, Tumor M2 PK antibody, Tumor M2-PK antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.