Plakophilin-3 (clone 23E3/4), anti-human, mouse, canine

Background:
Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The Human protein has a predicted molecular mass of 87 kD.

Clone: 23E3/4

Isotype: Mouse IgG2b

Source: 23E3/4 is a Mouse monoclonal IgG2b antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of Human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N–terminus (CFTPQSRRLRELPLAADALTF).

Reactivity: canine, human, mouse

Specificity: 23E3/4 reacts with an epitope loCated between residues 570-589 in Human plakophilin-3.

Form: Each vial contains 100ul purified monoclonal antibody in PBS containing 0.09% sodium azide.

Applications: Immunocytochemistry, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunoprecipitation, Western blotting

Storage: Store at 4C, or in small aliquots at -20C.