PLAP (clone F5C2 (C2)), anti-human

Source:
A BALB/c mouse was immunized with PLAP, purified from FS phenotype human placenta.
Fusion partner: X63-Ag8.P635.

Specifications:
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non tissue specific AP is found in kidney, liver and bone. F5C2 binds equally well to all common allelic variants (S,F, FS and I) of PLAP and to some variants of AP from normal human testis, while antibody H7E8 reacts with all variants of AP in normal human testis.

Species reactivity:
Positive: human.

Applications:
F5C2 can be used as epitope-defined marker in germ cell tumors and is applicable on frozen sections directly as well as on paraffin sections after a heat induced antigen retrieval step. F5C2 can be used at least as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes. F5C2 can also be used for immunosorbent purification and for radioimmuno-localization.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunoprecipitation: +
Paraffin sections: Citrate
Western blotting: +

Positive control: Human placenta.

References:
- Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)