PLAP (clone H7E8 (H7), Species: mouse), anti-human

Source:
A BALB/c mouse was immunized with PLAP, purified from FS phenotype human placenta.
Fusion partner: X63-Ag8.P653.

Specifications:
PLAP is a tissue specific, throphoblast-derived, 58 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallated glycoprotein, only found in humans, orangutans and chimpanzees, that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific APs in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98%homologous. Non tissue specific AP is found in kidney, liver and bone. H7E8 binds equally well to all common allelic variants (S,F, FS and I) of PLAP as to AP from normal human testis, while antibody F5C2 reacts with some samples of normal human testis only.

Species reactivity:
Positive: human.

Applications:
H7E8 can be used as epitope-defined marker in germ cell tumors and is applicable on frozen sections directly as well as on paraffin sections after a heat induced antigen retrieval step. H7E8 can be used both as coating as well as tracer antibody inthe same ELISA to detect PLAP in serum of S, F, FS and I phenotypes. H7E8 can also be used for immunosorbent purification and for radioimmuno-localization.:
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunoprecipitation: +
Paraffin sections: Citrate
Western blotting: +

Positive control: Human placenta.

References:
- Millan J.L. et al, Eur. J. Biochem. 136: 1-7 (1983)