Plectin polyclonal, anti-Human; strong cross-reactivity with bovine, mouse, xenopus laevis, fish

Quantity: 20 µl

Background:
Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes

Synonyms:
PLEC, HD1, PCN, EBS1, EBSO, PLTN, PLEC1, LGMD2Q, PLEC1b

Isotype: Guinea Pig Polyclonal GP21

Immunogen:
C-terminal C domain of human recomb. Plectin (aa 4367 - 4684)

Form: Stabilized Serum

Specificity:


Reactivity: Human; strong cross-reactivity with bovine, mouse, xenopus laevis, fish

Applications: Suitable for Frozen & Paraffin sections, Cytological material, Immunoblotting (Western)

Working DIlution: Immunohistochemistry: 1:100 to 1:200; Paraffin embedded tissue: 1:100; Western Blotting: 1:8,000 (ECL)

Molecular Weight:
Calculated MW 532,000 protein plectin, localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemi-desmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes). Human Glia cells are specifically detected.

Storage: 2-8C

References:
- Liu Ch.-G et al. (1996) Proc. Natl. Acad. Sci. USA 93: 4278-4283
- Schroder R et al. (1999) Eur J Cell Biol 78(4): 288-295