Plexin D1 (Cytoplasmic domain) polyclonal, anti-human, mouse, rat

Background:
Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.

Immunogen: Plexin D1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the cytoplasmic domain from human Plexin D1. This sequence is well conserved in rat and mouse Plexin D1, and has low homology to other Plexin family members.

Specificity: This antibody was affinity purified using Plexin D1 (Cytoplasmic) peptide (without carrier). The antibody detects 250, 215, and 195 kDa bands corresponding to the apparent molecular mass of Plexin D1 variants in human umbilical vein endothelial cells.

Buffer/Storage:
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.