Porcine CD40 ligand (CD40L) detection kit

Use: Used to detect the concentration of porcine CD40 ligand (CD40L) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-porcine CD40 ligand (CD40L) antibody (solid phase antibody), add the porcine CD40 ligand (CD40L) calibrator and the sample to be tested, then add the biotin-labeled antibody, after incubation and sufficient washing, add HRP-coupled avidin, after incubation and sufficient washing, remove the unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of porcine CD40 ligand (CD40L) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of porcine CD40 ligand (CD40L) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.