Porcine S100 calcium binding protein β (s-100β) detection kit

Use: Used to detect the concentration of porcine S100 calcium binding protein β (s-100β) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-porcine S100 calcium-binding protein β (s-100β) antibody (solid phase antibody), add the calibrator of porcine S100 calcium-binding protein β (s-100β) and the sample to be tested, then add the biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove the unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of porcine S100 calcium-binding protein β (s-100β) in the sample to be tested. By fitting the calibration curve, the concentration of porcine S100 calcium binding protein β (s-100β) in the sample can be calculated.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.