Porcine Ubiquitin (Ub) Detection Kit

Use: Used to detect the concentration of porcine ubiquitin (Ub) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled porcine ubiquitin (Ub) and purified anti-porcine ubiquitin (Ub) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies inhibit the competitive reaction with biotin-labeled porcine ubiquitin (Ub) and non-labeled antigens (calibrators or specimens). The binding amount of the antibody and biotin-labeled porcine ubiquitin (Ub) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled porcine ubiquitin (Ub), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated porcine ubiquitin (Ub) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated porcine ubiquitin (Ub)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of porcine ubiquitin (Ub) increases, the OD value gradually decreases, showing a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of porcine ubiquitin (Ub) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.