PP2Cα polyclonal, anti-human, mouse, rat

Background:
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.

Alternative Name:
PP2C-alpha, PPM1A, PPPM1A, Protein phosphatase 2C isoform alpha, EC 3.1.3.16, FLJ42306 antibody IA, MGC9201, Mpp alpha, PP2C alpha, PP2C-alpha, PP2CA, PP2Calpha, PPM 1A, PPM1A, PPM1A, PPPM1A, Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform, Protein phosphatase 1A, Protein phosphatase 1A magnesium dependent alpha, Protein phosphatase 2C alpha, Protein phosphatase 2C alpha isoform, Protein phosphatase 2C isoform alpha, Protein phosphatase IA, Protein phosphatase, Mg2+/Mn2+ dependent, 1A,

Application Dilution:
WB:1:500
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Specificity: PP2Cα polyclonal antibody detects endogenous levels of PP2Cα protein.

Immunogen:
Recombinant protein

MW: 42/35 kDa, additional band 55 kDa.

Swiss-Prot: P35813(Human) P49443(Mouse) P20650(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.