PPAR gamma polyclonal, anti-human

Quantity: 100 µg

Background:
The peroxisome proliferator-activated receptors(PPARs) are a group of three nuclear receptor isoforms, PPAR gamma, PPAR alpha, and PPAR delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements(PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. The PPAR gamma1 and gamma2 isoforms result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al.(2003) found that PPAR gamma is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases the expression of PPARgamma in endothelial cells and that loss of PPARgamma expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

Description:
Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor gamma (PPARG) detection. Tested with WB in Human.

Synonyms:
CIMT1 antibody, GLM1 antibody, HUMPPARG antibody, NR1C3 antibody, Nuclear receptor subfamily 1 group C member 3 antibody, OTTHUMP00000185032 antibody, OTTHUMP00000185036 antibody, PAX8/PPARG Fusion Gene antibody, Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody, Peroxisome proliferator activated receptor gamma 1 antibody, Peroxisome Proliferator Activated Receptor gamma antibody, Peroxisome proliferator-activated receptor gamma antibody, PPAR gamma antibody, PPAR-gamma antibody, PPARG antibody, PPARG_HUMAN antibody, PPARG1 antibody, PPARG2 antibody, PPARG3 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human PPAR gamma(45-62aa PHYEDIPFTRTDPVVADY), different from the rat and mouse sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.