PPT1 polyclonal (C-term), anti-human
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In stock
SKU
AC-AP2538b
Catalog Number: AC-AP2538b
Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB4707
Applications: WB, IHC-P
Datasheet
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Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB4707
Applications: WB, IHC-P
Datasheet
Request Information
Background:
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
Other Names:
Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
Target/Specificity:
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Antigen Region:
269-300
Antigen Type: Synthetic Peptide
Gene Name: PPT1
Gene ID: 5538
Primary Accession: P50897
NCBI Accession: NP_000301.1;NP_001136076.1
Other Accession: Q8HXW6
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
Other Names:
Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
Target/Specificity:
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Antigen Region:
269-300
Antigen Type: Synthetic Peptide
Gene Name: PPT1
Gene ID: 5538
Primary Accession: P50897
NCBI Accession: NP_000301.1;NP_001136076.1
Other Accession: Q8HXW6
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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