Pro-MMP-9 human active recombinant protein

Protein Family: Enzymes, Peptidases

Pathway and Disease: Immune System, Cancers

Alternate Names: Pro-MMP-9, Matrix metalloproteinase 9, gelatinase B, 92kD gelatinase, 92kD type IV collagenase precursor, CLG4B, GELB, MMP9Description:
Matrix metalloproteinases are a family of zinc- and calcium-dependant endopeptidases, which degrade extracellular matrix proteins. Matrix metalloproteinase 9 (MMP-9) is secreted as a 92-kDa zymogen (pro-MMP-9) cleaved into the 82-kDa active enzyme. MMP-9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain, and a hemopexin-like domain. MMP-9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth, and metastasis. Recombinant human Pro-MMP-9 comprises a 688 aa fragment (20-707) corresponding to the pro form of the protein minus the signal peptide and is expressed in E. coli with an amino-terminal hexahistidine tag.

Source: E. coli

Applications: E, WB

Accession No.: P14780

MW: 78.59 kDa

Sequence:
Proprietary

Purity:
Purity > 95% as determined by reducing and non-reducing SDS-PAGE, Bradford assay, Western blot and ELISA.

Format:
Each vial contains 0.01 mg protein (liquid) in PBS pH7.4, 50% glycerol. Final concentration 0.1-1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment.