Proprotein Convertase 2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
PCSK2(Proprotein Convertase, Subtilisin/Kexin-Type, 2), also known as PC2 or NEC2, is an enzyme responsible for the first step in the maturation of many neuroendocrine peptides from their precursors, such as the conversion of proinsulin to insulin intermediates. Seidah et al.(1991) mapped the NEC2 gene to human chromosome 20p11.2-p11.1. Ohagi et al.(1992) observed expression of the chloramphenicol acetyltransferase reporter fusions containing the putative promoter region in insulinoma cells but not in hepatoma cells, consistent with the known tissue-specific pattern of expression of the PC2 gene. Analysis of the level of CAT activity with several deletion mutants identified the region from -1100 to -539 from the translation start site as essential for PC2 promoter activity.

Description:
Rabbit IgG polyclonal antibody for Neuroendocrine convertase 2 (PCSK2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
KEX2 like endoprotease 2 antibody, KEX2-like endoprotease 2 antibody, NEC 2 antibody, NEC2 antibody, NEC2_HUMAN antibody, Neuroendocrine convertase 2 antibody, PC2 antibody, PCSK2 antibody, Prohormone convertase 2 antibody, Proprotein convertase 2 antibody, Proprotein convertase subtilisin/kexin type 2 antibody, SPC2 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Proprotein Convertase 2(110-125aa GYRDINEIDINMNDPL), identical to the related mouse and rat sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human Proprotein Convertase 2(110-125aa GYRDINEIDINMNDPL), identical to the related mouse and rat sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.