Prostaglandin E2 (PGE2) Detection Kit

Use: Used to detect the concentration of prostaglandin E2 (PGE2) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled prostaglandin E2 (PGE2) and purified anti-prostaglandin E2 (PGE2) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies and biotin-labeled prostaglandin E2 (PGE2) and non-labeled antigens (calibrators or specimens) inhibit the competitive reaction. The binding amount of the antibody and biotin-labeled prostaglandin E2 (PGE2) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less the antibody and biotin-labeled prostaglandin E2 (PGE2), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated prostaglandin E2 (PGE2) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated prostaglandin E2 (PGE2)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of prostaglandin E2 (PGE2) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of prostaglandin E2 (PGE2) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.