Prostaglandin F2α (PGF2a) Detection Kit

Use: Used to detect the concentration of prostaglandin F2α (PGF2a) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled prostaglandin F2α (PGF2a) and purified anti-PGF2α (PGF2a) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibody inhibits the competitive reaction with biotin-labeled prostaglandin F2α (PGF2a) and non-labeled antigen (calibrator or specimen). The binding amount of antibody and biotin-labeled prostaglandin F2α (PGF2a) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of antibody and biotin-labeled prostaglandin F2α (PGF2a), and vice versa. After the reaction is balanced, solid-phase antibody-biotinylated prostaglandin F2α (PGF2a) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated prostaglandin F2α (PGF2a)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of prostaglandin F2α (PGF2a) increases, the OD value gradually decreases and shows a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of prostaglandin F2α (PGF2a) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.