PRPF19 polyclonal, anti-human, mouse, rat

Background:
The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5‘ splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

Alternative Name:
Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, hPso4, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, PRPF19I, NMP200, PRP19, SNEV

Application Dilution:
WB: 1:500~1:1000

Specificity: PRPF19 polyclonal antibody detects endogenous levels of PRPF19 protein.

Immunogen:
Synthetic peptide, corresponding Human PRPF19.

MW: ~ 55 kDa

Swiss-Prot: Q9UMS4

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.