PSMB8 polyclonal, anti-human, mouse, rat

Background:
The eukaryotic multi-catalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched-chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7A and LMP7B, also designated LMP7-E1 and E2, in several tissues.

Alternative Name:
PSMB8, LMP7, PSMB5i, RING10, Y2, ALDD, D6S216, D6S216E, Large multifunctional peptidase 7, Large multifunctional protease 7, LMP 7, LMP7, Low molecular mass protein 7, Low molecular weight protein 7, Macropain subunit C13, MGC1491, Multicatalytic endopeptidase complex subunit C13, NKJO, OTTHUMP00000062981, Protease component C13, Proteasome (prosome macropain) subunit beta type 8, Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7), Proteasome beta 8 subunit, Proteasome catalytic subunit 3i, Proteasome component C13, Proteasome related gene 7, Proteasome subunit beta 5i, Proteasome subunit beta 8, Proteasome subunit beta type 8, Proteasome subunit beta type, Proteasome subunit beta type-8, Proteasome subunit beta-5i, Proteasome subunit Y2, PSB8, PSMB 8, PSMB5i, PSMB8, Really interesting new gene 10 protein, RING 10, RING10, Y2,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: PSMB8 polyclonal antibody detects endogenous levels of PSMB8 protein.

Immunogen:
Recombinant protein

MW: 25 kDa

Swiss-Prot: P28062(Human) P28063(Mouse) P28064(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.