PSMB9 polyclonal, anti-human, mouse

Background:
The eukaryotic multicatalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7-E1 and E2, in several tissues.

Alternative Name:
PSMB9, LMP2, PSMB6i, RING12, Beta1i, Large multifunctional peptidase 2, Large multifunctional protease 2, LMP 2, LMP2, Low molecular mass protein 2, Macropain chain 7, MGC70470, Multicatalytic endopeptidase complex chain 7, OTTHUMP00000062982, Proteasome (prosome macropain) subunit beta type 9, proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2), Proteasome beta 9 subunit, Proteasome catalytic subunit 1i, Proteasome chain 7, Proteasome related gene 2, Proteasome subunit beta 6i, Proteasome subunit beta type 9, Proteasome subunit beta type-9, Proteasome subunit beta-1i, PSB9, PSMB 9, PSMB6i, PSMB9, Really interesting new gene 12 protein, RING 12, RING12, RING12 protein,

Application Dilution:
WB:1:500-1:1,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: PSMB9 polyclonal antibody detects endogenous levels of PSMB9 protein.

Immunogen:
Recombinant protein

MW: 23 kDa

Swiss-Prot: P28065(Human) P28076(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.