PSMD12 Polyclonal Antibody

Background:
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research Area: Cell Biology

Synonyms:
26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit p55, 26S proteasome regulatory subunit RPN5, MGC75406, p55, proteasome (prosome, macropain) 26S subunit, non ATPase12, Proteasome 26S non ATPase subunit 12 isoform 2, PSD12, PSMD12, Rpn5

Immunogen: Fusion protein of human PSMD12

Swissprot: O00232
Gene Accession: BC019062

Calculated MW: 53 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.9 mg/mL
Dilution: WB 1:1000-1:5000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.