PTGER4 293 Cell Lysate
€447.00
In stock
SKU
400374
Protein Family: Receptors and Channels
Pathway & Disease: Cancers, Endocrine System, Signal Transduction, Signaling Molecules and Interaction
Description:
Antigen standard for prostaglandin E receptor 4 (subtype EP4) (PTGER4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210932, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: PGE receptor, EP4 subtype, prostanoid EP4 receptor, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, PTGER4, EP4, EP4R, MGC126583
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: E, WB, IP
Accession No: NP_000949
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Pathway & Disease: Cancers, Endocrine System, Signal Transduction, Signaling Molecules and Interaction
Description:
Antigen standard for prostaglandin E receptor 4 (subtype EP4) (PTGER4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC210932, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Alternate Names: PGE receptor, EP4 subtype, prostanoid EP4 receptor, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, PTGER4, EP4, EP4R, MGC126583
ORF clone URL:
Format: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).
Applications: E, WB, IP
Accession No: NP_000949
Application Notes: WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Storages: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
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