PUF60 polyclonal, anti-human, mouse, rat
€388.00
In stock
SKU
BS8373
Background:
Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression. The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity. FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators. FIR blocks activator-dependent, but not basal transcription through TFIIH. FIR shares identity with seven in absentia (siah) binding protein 1. FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary .
Alternative Name:
Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, FBP-interacting repressor, Ro-binding protein 1, RoBP1, Siah-binding protein 1, Siah-BP1, PUF60, FIR, ROBPI, SIAHBP1
Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100, IP: 1:20 - 1:50
Specificity: PUF60 polyclonal antibody detects endogenous levels of PUF60 protein.
Immunogen:
Recombinant full length Human PUF60.
MW: ~ 60 kDa
Swis Prot.: Q9UHX1
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc. The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression. The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity. FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators. FIR blocks activator-dependent, but not basal transcription through TFIIH. FIR shares identity with seven in absentia (siah) binding protein 1. FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary .
Alternative Name:
Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, FBP-interacting repressor, Ro-binding protein 1, RoBP1, Siah-binding protein 1, Siah-BP1, PUF60, FIR, ROBPI, SIAHBP1
Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100, IP: 1:20 - 1:50
Specificity: PUF60 polyclonal antibody detects endogenous levels of PUF60 protein.
Immunogen:
Recombinant full length Human PUF60.
MW: ~ 60 kDa
Swis Prot.: Q9UHX1
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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