pyruvate dehydrogenase (lipoamide α 1 (clone 3H2-F8-B5), anti-human, mouse

Background:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms: PDHA, PDHAD, PDHCE1A, PHE1A

Cellular Location: Mitochondrion matrix

Immunogen:
Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.

Gene Symbol: pyruvate dehydrogenase (lipoamide) α 1

Gene ID: 5160

Swiss prot: P08559

Calculated MW: 43kDa

Recommended dilution:
WB 1:1000, IHC 1:100

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.